Project

Part:BBa_K3982026:Design

Designed by: Akankshya Ramkrishna Sahu   Group: iGEM21_IISER_Berhampur   (2021-10-01)


CODE M Standard Construct S1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1457
    Illegal PstI site found at 247
    Illegal PstI site found at 413
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1457
    Illegal PstI site found at 247
    Illegal PstI site found at 413
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1457
    Illegal BamHI site found at 1051
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1457
    Illegal PstI site found at 247
    Illegal PstI site found at 413
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1457
    Illegal PstI site found at 247
    Illegal PstI site found at 413
    Illegal AgeI site found at 707
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Parts from CODE M Construct C1 were used with RE sites Xba1 and Spe1 to design this part.

Source

Addgene #112502

References